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Fig. 4 Zebrafish treated with morpholino against rrp12 and noc3l. (A) Stage of development at day 7 after morpholino injection in the zebrafish embryos. Top and middle left: uninjected animals and embryos injected with control MO show normal development, normal head size, and straight body. Lower left: embryos injected with noc3l ATG MO also present normal development, size, and body strength. Right: embryos injected with rrp12 ATP MO presented different degrees of abnormalities on day 7, which were consistent with delayed development and significant reduction in head size (10% of embryos, top right), crimping, and developmental delay (77% of embryos, middle right), as well as severe crimping and developmental abnormalities (13% of embryos, lower right). (B) Survival was significantly reduced by day 6 in the rrp12-injected embryos, whereas it was only mildly reduced (to 80%) in noc3l ATG MO-injected fish. Control MO did not alter survival compared to uninjected animals.