Figure 5.

Figure 5.

Sqstm1, optineurin and atg13 are not involved in autophagic response to S. pneumoniae. (A) Representative confocal images of sqstm1^+/+optn^+/+ and sqstm1^−/−optn^−/− double mutant CMV:GFP-Lc3 larvae infected systemically with approximately 1600 CFU of mKate2-labeled D39 Δcps S. pneumoniae and fixed at 2 hpi Scale bars: 10µm. (B) Quantification of Lc3 associations with intracellular S. pneumoniae within infected phagocytes of fixed CMV:GFP-Lc3 sqstm1^+/+optn^+/+ and sqstm1^−/−optn^−/− double mutant larvae. Data are shown as mean±standard deviation (SD). Data obtained from two independent experiments. (C) Numbers of viable colony forming units (CFUs) over time of sqstm1^+/+optn^+/+ and sqstm1^−/−optn^−/− double mutant larvae. Data obtained from two independent experiments. (D) Representative confocal images of control and atg13 knockdown (right panel) CMV:GFP-Lc3 larvae infected systemically with approximately 1600 CFU of mKate2-labeled D39 Δcps S. pneumoniae and fixed at 2 hpi Scale bars: 10µm. (E) Quantification of Lc3 associations with intracellular S. pneumoniae within infected phagocytes of fixed CMV:GFP-Lc3 control and atg13 knockdown larvae. Data are shown as mean±standard deviation (SD). n≥15 zebrafish larvae were analyzed.