Figure 3.

Figure 3.

ROS-dependent autophagic response contribute to clearance of S. pneumoniae. (A, D) Representative confocal images of control and indicated gene knockdown (A – cyba, D - rubcn) CMV:GFP-Lc3 larvae infected systemically with approximately 1600 CFU of mKate2-labeled D39 Δcps S. pneumoniae and fixed at 2 hpi. Scale bars: 10 µm. (B, E) Quantification of Lc3 associations with intracellular S. pneumoniae within infected phagocytes of fixed CMV:GFP-Lc3 control and indicated gene knockdown (B – cyba, E - rubcn) larvae at 2 hpi. Data are shown as mean ± standard deviation (SD). Data obtained from three independent experiments. (C, F) Numbers of viable colony forming units (CFUs) over time of control and indicated gene knockdown (C – cyba, F - rubcn). Data obtained from three independent experiments.