Figure 1.

Figure 1.

The autophagic response occurs during systemic infection with pneumococci. (A) Representative confocal images of CMV:GFP-Lc3 larvae infected systemically with approximately 1600 CFU of mKate2-labeled D39 Δcps S. pneumoniae and fixed at 2, 4 and 7 hpi. Scale bars: 10 µm. Arrowheads indicate Lc3-positive vacuoles containing bacteria. (B) Quantification of Lc3 associations with intracellular S. pneumoniae within infected phagocytes of fixed CMV:GFP-Lc3 at 2, 4 and 7 hpi. Data are shown as mean ± standard deviation (SD). Data obtained from two independent experiments (C) Representative confocal images of CMV:GFP-Lc3 mpeg:mCherry-F dual transgenic larvae infected systemically with approximately 1600 CFU of Alexa Fluor 647-stained D39 Δcps S. pneumoniae and fixed at 2 hpi.