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Figures for Chen et al., 2025
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FIGURE 2

Spatiotemporal expression pattern of ddx3xa by whole-mount in situ hybridization and qRT-PCR analysis. (A–J) Spatiotemporal expression of ddx3xa at various developmental stages visualized by whole-mount in situ hybridization. Scale bar: 250 μm (applies to A–J). (A) 0.2 hpf; (B) 12 hpf; (C) 24 hpf; (D) 36 hpf; (E) 48 hpf; (F) 72 hpf; (G) Dorsal view, 24 hpf; (H) Dorsal view, 36 hpf; (I) Dorsal view, 48 hpf; (J) Dorsal view, 72 hpf. (K) Quantitative real-time PCR (qRT-PCR) analysis of relative ddx3xa transcript levels at various developmental time points (0.2, 3, 5.3, 8, 12, 24, 36, 48, 72, 96, 196 hpf). Expression levels were normalized to the 0.2 hpf time point. (n = 3; **P < 0.01, ***P < 0.001).

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