Fig. 2

Fig. 2 prpf4 deficiency reduces the number of erythrocytes in zebrafish.

A, B The number of erythrocytes in the CHT region of siblings and prpf4^−/− embryos was assessed using confocal microscopy in Tg(gata1:DsRed) transgenic zebrafish. Scale bar: 100 µm. C, D FACS analysis of GATA1⁺ erythrocytes from siblings and prpf4^−/− mutants at 48 hpf. C In prpf4 mutants, the proportion of GATA1⁺ erythrocytes was reduced (30 embryos per group). D Quantitative analysis of GATA1⁺ erythrocyte numbers in individual embryos. E Cell cycle analysis of GATA1⁺ erythrocytes from siblings and prpf4^−/− mutants at 48 hpf (30 embryos per group). F Quantification of the percentage of cells in each cell cycle phase. G, H Confocal microscopy analysis of apoptosis in GATA1⁺ erythrocytes in the CHT region of siblings and prpf4^−/− embryos at 54 hpf. G Immunofluorescence staining showing GATA1 (red), active Caspase-3 (green), and DAPI (blue). Arrows indicate apoptotic GATA1⁺ cells. Scale bar: 50 µm. H Quantitative analysis shows a significantly higher proportion of apoptotic GATA1⁺ cells in the CHT region of prpf4^−/− embryos compared with siblings.