ZFIN Image: Ren et al., 2025, Figure 6.

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Figure Caption

Figure 6.

The deficiency of RPGR^ORF15 leads to lysosome dysfunction in RPE-1 cells. (A,B) Protein levels and quantification of phagocytosis related-genes in NC and siRPGR^ORF15 RPE-1 cells (n = 3 biological replicates). (C) Immunostaining of early endosomes (Rab5a), Rab7a, LAMP1, and CTSB in NC and siRPGR^ORF15 RPE-1 cells. Scale bar = 10 µm. (D,E) Lysotracker staining and quantification of signal dots per cell in NC and siRPGR^ORF15 RPE-1 cells (n = 28). Scale bar = 10 µm. Data are presented as mean ± SD. Statistical significance is indicated as not significant (ns), *P < 0.05, **P < 0.01, ***P < 0.001.

Acknowledgments

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