Fig. 2

Fig. 2 Locomotor activity during the light-dark test (LDT) in larval ube3a and fmr1 zebrafish.

(A) Representative movement traces of ube3a WT and mutant larvae during alternating light and dark phases (Left panel); Mean distance travelled across all light and dark phases in ube3a WT and mutant larvae by 10-s time bins (Middle panel); Distance travelled (mm) and velocity (mm/s) between WT (N = 12) and mutants (N = 11); unpaired t test. (B) Representative movement traces of fmr1 WT and mutant larvae during alternating light and dark phases (Left panel); Mean distance travelled across all light and dark phases in fmr1 WT and mutant larvae by 10-s time bins (Middle panel); Distance travelled (mm) and velocity (mm/s) between WT (N = 12) and mutants (N = 12); unpaired t test. (C) Genotype: stimulus effect calculated as the dark-light effect per larvae in each genotype; unpaired t tests. The data are presented as boxplots with whiskers (IQR-based). Exact P values are provided in the Figures.