|
Figure 8
hMR and zfMR activities of waste water samples
(A–C) Mineralocorticoid activities of waste water influent and effluent of two urban sites, A and B, were tested in the UG5LN GAL4-hMR and UG5LN-GAL4-zfMR cells. Firstly, influent and effluent of the two sites were tested at the maximal concentration of 3 REF for influents and 6 REF for effluents (A). Secondly, serial dilutions of waste water influents A (B) and B (C) were tested in UG5LN-GAL4-hMR, UG5LN-GAL4-zfMR, and UG5LN-GAL4-hMR (T870L) cells. Sample concentration data are expressed in REF. Results are expressed as the percentage of the maximum luciferase activity induced by 10−8 M aldosterone. The curves are presented as a non-linear regression; log (REF) versus response.
Data are presented as means ± SD values. n (replicates per experiment) = 4 per group.