Figure 1

Figure 1 BICD2 is located at the mother centriole.

(A) 3D-SIM immunostaining images of RPE-1 cells stained with BICD2 antibody in conjunction with antibodies against PCM1, Centrin, C-Nap1, or CEP164. RPE-1 cells were cultured in normal serum medium. Scale bar, 1 μm. (B) Multi-color confocal images of RPE-1 cells stained with BICD2 antibody, along with Centrin and CEP164 antibodies. RPE-1 cells were cultured in normal serum medium. Scale bar, 1 μm. (C) Schematic illustration of BICD2 localization at the mother centriole. (D) Schematic diagrams of full-length BICD2 and BICD2 truncation mutants. (E) Immunostaining images of RPE-1 cells infected with lentiviruses carrying the indicated plasmids for 48 h, treated with puromycin for an additional 48 h, and stained with antibodies against Flag and CEP164. RPE-1 cells were cultured in normal serum medium. DNA was stained with DAPI. Scale bars, 5 μm (two columns on the left), 1 μm (three columns on the right). Source data are available online for this figure.