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Fig. 5

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Figures for Li et al., 2025
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Fig. 5 Red fluorescent protein (RFP) accumulation and its implications in the atrioventricular (AV) valve of tomato zebrafish.

A Bright-field image (left) and RFP fluorescence (right) from WT and tomato zebrafish hearts. Scale bar, 1 mm. B Illustration of RFP expression (shown in red) in the AV and bulboventricular (BV) valves of tomato zebrafish. A atrium, V ventricle, AV valve atrioventricular valve, BV valve bulboventricular valve. C Immunohistochemical detection of RFP (red) expression in the AV valve region of WT and tomato zebrafish at 3 months post-fertilization (mpf). DAPI (blue) staining of cell nuclei. White dotted lines: atrioventricular (AV) valves. Scale bar, 50 μm. Dtph2 expression in the AV valve region of two WT zebrafish was detected by section in situ hybridization at 3 mpf. Black dotted lines: atrioventricular (AV) valves. Scale bar, 50 μm. E Schematic of Cre mRNA injection. Black arrows: heart position. Scale bar, 10 mm.

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