FIGURE 5

FIGURE 5

Trβ2 upregulation precedes lws1 activation and aligns with lws2 downregulation). (A) RT-qPCR analysis of wildtype and trβ2 GOF transgenic fish in a time-series experiment, treated with 100 nM T3, to assess the temporal dynamics of lws1, lws2, and trβ2 mRNA expression. (B) Line graph showing the kinetics of fold change in lws1, lws2, and trβ2 mRNA expression over time (hours post treatment, hpt). Scatter plots and line graphs represent fold changes in transcript expression (2^-ddCT). Each point in the scatter plot corresponds to a biological replicate (n ≥ 6). P-values were determined by comparing ddCT values across groups using Kruskal–Wallis one-way ANOVA with post hoc Dunn’s correction. Statistical significance is indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. WT = wildtype; TG = transgenic overexpressing trβ2.