Figure 4

Figure 4

Role of ezh2 in the differentiation of cerebellar granule and Purkinje cells: (A) whole-mount RNA in situ hybridization of the brain region of ezh2^+/+ and ezh2^−/− siblings at 3 and 5 dpf, as indicated, to detect the expression of neurod1, a marker expressed in immature granule cells, slc17a7a, a marker of differentiated granule cells, and pvalb7, a marker of differentiated Purkinje cells. The yellow arrows emphasize expression profile differences between ezh2^+/+ and ezh2^−/− larvae. Scale bar is 200 µm; and (B) schematic representation of some zebrafish brain structures at 5 dpf. The retina is shown in green, the habenula in black, the torus longitudinalis in brown, the corpus cerebelli (Cce) in light blue, the lobus caudalis (LCa) cerebelli in dark blue, the valvula cerebelli (Va) in purple, and the eminentia granularis (EG) in yellow.