ZFIN Image: Malavašič et al., 2025, Fig. 4

Image

Figure Caption

Fig. 4

Mineralization and ALPL expression are abolished in MPP7-KO cells. Wild-type (WT) and MPP7-KO HOS cells were cultured in osteogenic differentiation medium and analyzed every 7 days up to day 35. A Representative light microscopy images of differentiating WT and MPP7-KO cells stained with Alizarin Red S to detect calcium deposits (red). Scale bars represent 1 mm. B Relative mRNA expression of alkaline phosphatase (ALPL) as determined by RT-qPCR. C Quantification of Alizarin Red S staining by spectrophotometry. Data represent mean ± SD of three independent biological replicates (N = 3), *P ≤ 0.05; **P ≤ 0.01

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Calcif. Tissue Int.