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Fig. 6 col4a1Δ20 mutant larvae exhibit a locomotor deficit. A. Schematic demonstrating the white light display performed by Daniovision software. B. Representative swimming tracks recorded from an aerial view using Daniovison software. C. col4a1+/Δ20 (HET) and col4a1Δ20/Δ20 (HOM) larvae exhibit a decrease in distance travelled (mm) compared to compared to col4a1+/+ (WT) siblings. D. col4a1+/Δ20 and col4a1Δ20/Δ20 larvae exhibit decreased velocity compared to col4a1+/+ siblings. E. col4a1Δ20/Δ20 larvae spent less time active than col4a1+/+ siblings. Time active is calculated as a percentage of total time spent moving during a 10-minute recording. C-E. Data were analysed with a one-way ANOVA and presented as mean, and data points represent measurements from individual larvae, col4a1+/+ n = 77, col4a1+/Δ20 n = 131, col4a1Δ20/Δ20 n = 64 from 4 independent replicates with larvae genotyped afterwards. Asterisks signify means significantly different at the 5 % level using the Tukey HSD test with * P ≤ 0.05, **P ≤ 0.01 and ns denoting no significant difference.