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Fig. 2 Survival rates and genotypes of embryos derived from inter-crossing grhl3+/− zebrafish of all three lines. (A) Comparison of survival rate of embryos derived from inter-crossing the grhl3+/−(+14bp), grhl3+/−(−31bp), and grhl3+/−(Δ1-4) lines. (B) Comparison of the phenotypic incidence of class 1, class 2, and class 3 phenotypes in grhl3−/−(+14bp), grhl3−/−(−31bp), and grhl3−/−(Δ1-4) embryos at 72 hpf. (C) Percentage of heterozygous, homozygous null, and WT larvae present at 5 dpf in a representative sample of 39–40 embryos. (D–F) Representative genotyping experiments showing the presence of wild type (+/+), heterozygous (+/−), and homozygous null (−/−) embryos of grhl3(+14bp), grhl3(−31bp), and grhl3(Δ1-4) lines.