ZFIN Image: Xiang et al., 2025, Fig. 4

Image

Figure Caption

Fig. 4

mRNA sequencing results of primary osteoblasts from CT group patients before and after overexpression of miRNA-130b-3p. Volcano plot showing gene expression changes, with 460 genes upregulated and 114 genes downregulated Heatmap of the top 20 genes with the most significant differential expression c. KEGG enrichment analysis bubble plot, displaying the top 15 pathways d. KEGG enrichment analysis circular plot e. Prediction of potential target genes of miRNA-130b-3p using the miRTarBase and TargetScan7 databases f. Interaction between miR-130b-3p and two predicted binding sites in the 3’-UTR of IGF1. The miR-130b-3p seed sequence is highlighted in red g. Dual-luciferase assay showing IGF1 as a target gene of miRNA-130b-3p

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell. Mol. Life Sci.