ZFIN Image: Xiang et al., 2025, Fig. 1

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Fig. 1

Impaired osteogenic differentiation potential of primary osteoblasts from AIS patients.a. left: Immunohistochemical staining of OPN in the facet joints of the spine from non-AIS control patients (up) and AIS patients (down). Mid: Alkaline Phosphatase staining of primary osteoblasts after 3 days of osteogenic differentiation from non-AIS control patients (up) and AIS patients (down). Right: Alizarin Red S staining of primary osteoblasts after 14 days of osteogenic differentiation from non-AIS control patients (up) and AIS patients (down) b. Quantification of immunohistochemical staining results (n = 10) c. Quantification of Alkaline Phosphatase staining results (n = 10) d. Quantification of Alizarin Red S staining results (n = 10) e. Western blot (WB) results for primary osteoblasts from non-AIS control patients (left) and AIS patients (right) after 3, 7, and 14 days of osteogenic differentiation (n = 4) f. Quantification of RUNX2 protein expression g. Quantification of OCN protein expression **:p<0.01; ***:p<0.001

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