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Fig. 4 No iridophores were observed in tfec−/− mutants at 5 and 10 dpf. (A-F) Many iridophores and melanophores were observed on the iris and yolk sac, respectively, in wild-type fish (A-C), while no iridophores and a small number of melanophores were observed on the iris and yolk sac, respectively, in tfec−/− mutants (D-F). (G-L) Iridophores were observed on the iris, operculum, peritoneum and swim bladder of wild-type fish (G-I), while no iridophores were observed in tfec−/− mutants which displayed transparent phenotype with the gill filaments, heart, swim bladder, intestine, gallbladder, and liver visible from outside (J-L). Gf, Gill filaments; He, heart; Ga, gallbladder; In, intestine. Scale bar in A-J, 1 mm.
Reprinted from New biotechnology, 89, Liang, G., Lu, B., Dai, S., Li, M., Yao, J., Liu, H., Liu, X., Liu, X., Wang, D., Creation of colorless transparent tilapia using CRISPR/Cas9 mediated multi-gene mutation, 163-176, Copyright (2025) with permission from Elsevier. Full text @ N. Biotechnol.