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Fig. 1 Hdac11 negatively regulates pdcd6 and apoptosis in zebrafish cells. (a, b) ZF4 cells were pre-treated with 1 µM JB3-22 or DMSO for 2 h, or transfected with pcDNA3.1(-) (Vector) or pcDNA3.1(-)-flag-hdac11-WT (OE-hdac11) for 8 h, then cultured at 28 °C–18 °C (cold stress) for 24 h. Apoptosis was detected with Annexin V-FITC/PI staining. (c) Relative mRNA level of hdac11 in ZF4 cells after transfection with pcDNA3.1(-)-flag-hdac11-WT for 24 h. (d) Relative mRNA level of pdcd6 in ZF4 cells after treatment with 1 µM JB3-22 for 24 h or transfected with pcDNA3.1(-)-flag-hdac11-WT (OE-hdac11) for 36 h. (e, f) Relative mRNA level of hadc11 and pdcd6 in WT and hdac11−/− zebrafish liver. Data are represented as mean ± SD of three independent experiments, and P values are calculated using Student’s t-test (n.s. P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).