ZFIN Image: Zhang et al., 2025, Fig. 2

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Figure Caption

Fig. 2 Effects of BPA and its analogs on the number of innate immune cells in zebrafish embryos at 72 hpf. (A) The number of neutrophils in Tg (lyz: DsRED2) zebrafish embryos; A1, representative fluorescence images of neutrophils from the control and 1000 μg/L bisphenol treatment groups; the white rectangle shows the caudal hematopoietic tissue (CHT) area in the tail of the embryos; A2, statistical maps of the number of neutrophils in the CHT region. (B) The number of macrophages in wild-type AB zebrafish embryos; B1, representative images of neutral red-labeled macrophages from the control and 1000 μg/L bisphenol treatment groups; B2, statistical analysis of macrophages. The results are shown as the mean ± SDs (n ≥ 20). Significant differences between bisphenol treatments and controls are denoted by asterisks (*p < 0.05, ** p < 0.01, *** p < 0.001). Scale bars represent 500 μm (A1) and 200 μm (B1).

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