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Fig. 7 ISR Inhibition reduces islet macrophage number in prediabetic female NOD mice. Prediabetic 6-week-old female NOD mice were treated with ISRIB (2.5 mg/kg) for two weeks. A Identified protein levels in the insulitic area. B Identified protein levels in the insulin-positive area; n = 10–12 ROI from 2 mice per group. C Representative images of pancreata from ISRIB-treated NOD mice stained for F4/80 (brown), and counterstained with hematoxylin (blue). Dotted lines indicate islets and arrows indicate macrophages. Scale bars: 100 μm. D Quantification of F4/80 + cells in islet area; n = 4–5 biological replicates. E Quantification of F4/80 + cells in islets with insulitis. F Quantification of F4/80 + cells in islets without insulitis. Statistical tests: Unpaired t-tests: A-B, D-F. Data are represented as mean ± SEM