|
Fig. 2 scRNA-seq indicates a hair cell differentiation fate switch in prdm1a mutants. a UMAP plot of prdm1a-/- and sibling scRNA-seq, from central support cells to mature hair cells, colored by cell type. Black arrows indicate developmental trajectory. b UMAP plot of prdm1a-/- and sibling scRNA-seq, from central support cells to mature hair cells, colored by genotype. c Feature plot of prdm1a expression in the scRNA-seq hair cell lineage sub-clustering. d Heatmap of selected genes upregulated in prdm1a-/- hair cells, and non-differentially expressed hair cell marker genes. e–h Feature plots of pvalb9, ckbb, s100a1 and tmc2a from the hair cell trajectory sub-clustered scRNA-seq. i–p HCRs of pvalb9, ckbb, s100a1 and tmc2a in 5 dpf sibling and prdm1a-/- neuromasts. Representative images of two independent experiments. q–t HCRs of pvalb9, ckbb, s100a1 and tmc2a in the lateral crista of the ear in Tg(myo6b: H2B-mScarlet) larvae, with hair cells are labeled in magenta. Scale bars= 10 µm. Representative images of two independent experiments