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Figure Caption
Fig. 4 Apoptosis detection in zebrafish after DBI treatment. (A) Representative images of 2 week-old pax3a:EGFP zebrafish without tumors treated with DBI, followed or not by illumination. (B) Sibling zebrafish with rhabdomyosarcoma tumors treated with DBI, followed or not by illumination. Dashed squares indicate the illuminated areas. Apoptotic cells were detected with TUNEL staining (red), and nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (C) Quantification of TUNEL-stained area in healthy and tumor regions, treated with DBI, followed or not by illumination. p < 0.001(***).
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