Fig. 5

Fig. 5

The impact of IS20 on the expression of EGFR and its downstream signaling proteins in K562 (A) and U-251 (B) cells. The results of protein expression are presented as the mean ± SD of six independent experiments (n = 6). Immunoblots shown are representatives from these independent experiments. The displayed blots were cropped, and the full-length blots are shown in Fig. S6. Statistical significance is presented above as *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 relative to the respective control, statistical analysis was done using one-way ANOVA with Bonferroni’s posthoc test (for Western Blot) or Dunn–Šidák correction test (for Lumit Immunoassay).