|
Fig. 5 Knockdown of igfbp1a affects intestinal regeneration after Mtz-induced damage. (A) Experimental design for morpholino-mediated knockdown experiments. (B) GFP+ intestinal area measurements in control and igfbp1a morphants during ablation and recovery. Pooled data from 2 independent experiments are shown (1 dot = 1 larva). (C) Representative confocal images of the intestines of analyzed groups. Scale bar = 200 µm. (D) BrdU incorporation in control and igfbp1a morphants during recovery (5dpf). Scale bar = 200 µm in whole-intestine pictures and 100 µm in zoomed area. Pooled data from 2 independent experiments (1 dot = 1 larva). (E) Intestinal permeability of control and igfbp1a morphants during recovery (5dpf) assessed by oral gavage of rhodamine-dextran. Normalized mean fluorescence in the trunk was measured. Scale bar = 200 µm. Pooled data from 2 independent experiments (1 dot = 1 larva). Different symbol shapes denote independent experiments in B, D, and E. Two-way ANOVA with Tukey’s post-tests were performed in B, D, and E. *p < 0.05; ***p < 0.001.