ZFIN Image: Petzold et al., 2025, Fig. 4.

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Figure Caption

Fig. 4.

Stabilisation of Hif1/2α rescues haemogenic endothelium induction and HSPC specification in cx41.8^tq/tq mutants. (A) Quantification of aortic gata2b signal (in situ hybridisation) at 23 hpf in cx41.8^tq/tq control embryos and cx41.8^tq/tq embryos supplemented with CoCl₂ or DMOG. (B) Quantification of aortic runx1 signal (in situ hybridisation) at 28 hpf in cx41.8^tq/tq control embryos and cx41.8^tq/tq embryos treated with CoCl₂ or DMOG. (C) In situ hybridisation and quantification of gata2b at 23 hpf in WT control embryos and cx41.8^tq/tq embryos injected with either control- or vhl-MO. (D) In situ hybridisation and quantification of runx1 at 28 hpf in control-MO or vhl-MO injected wild-type or cx41.8^tq/tq mutant embryos. Statistical significance was calculated using a Chi-squared test. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Scale bars: C, 100 μm, D, 200 μm.

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