Fig. 1.

Fig. 1.

The I203F mutation in Cx41.8 results in a defect in haemogenic endothelium induction and HSPC specification. (A) The leo^tq270 (cx41.8^tq/tq) mutant possesses an I203F change in the fourth transmembrane domain. (B) Sanger sequencing shows an A-to-T base change in the cx41.8^tq/tq mutant. (C) In situ hybridisation and quantification of gata2b in cx41.8^tq/tq mutants and controls at 24 hpf. (D) In situ hybridisation and quantification of runx1 in cx41.8^tq/tq mutants and controls at 24 hpf. (E) Quantification of cmyb:GFP⁺ haemogenic endothelial (HE) cells and HSPCs in cx41.8^tq/tq and control embryos between 28 and 50 hpf. Statistical significance was calculated using either a Chi-squared test (C and D) or an unpaired t-test (E). *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. Scale bars: 200 μm (C and D). Created in BioRender by Petzold, T., 2025. https://BioRender.com/97nw7ib. This figure was sublicensed under CC-BY 4.0 terms.