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Fig. 7 Immunofluorescence assay (day 9) and bacterial infection analysis (day 6) in irradiated mice. Effect of Mulberroside C (MC, 2–8 mg/kg) or granulocyte colony-stimulating factor (G-CSF, 25 μg/kg) in irradiated KM mice. (A) Representative immunofluorescence images showing Ki67+Ly6G+ cells in the bone marrow and spleen (Ki67: Green; Ly6G: Red) (scale bar, 10 μm). The histograms illustrate the percentage of Ki67+Ly6G+ cells (B) in bone marrow and (C) in spleen. Three technical replicates (fields of view) were analyzed from one mouse. (D) Bacterial killing assay. (E) Quantification of bacteria killed in each group. n = 3. Data are expressed as the mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 versus the model group.