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Fig. 3 Effects of Mulberroside C (MC) on leukopenia in zebrafish. (A) Timeline of the zebrafish intervention protocol. Effect of granulocyte colony-stimulating factor (G-CSF, 25 μg/L) or MC (5–20 μM) in irradiated or cyclophosphamide-treated transgenic zebrafish Tg(mpeg1: EGFP) and Tg(lyz: DsRed). (B) Toxicity of MC (2.5–80 μM) on zebrafish survival and development at 2 days postfertilization (2 dpf). n = 30. (C) Fluorescent imaging of mpx: EGFP-positive cells in irradiated Tg(mpeg1: EGFP) transgenic zebrafish at 6 dpf. (D) Quantification of mpx:EGFP-positive cells in each group. n = 12. (E) Fluorescent imaging of mpx:EGFP-positive cells in cyclophosphamide-treated Tg(mpeg1: EGFP) zebrafish at 6 dpf. (F) Quantification of mpx:EGFP-positive cells in each group. n = 6. (G) Fluorescent imaging of lyz:DsRed-positive cells in irradiated Tg(lyz: DsRed) transgenic zebrafish at 6 dpf. (H) Quantification of lyz: DsRed-positive cells in each group. n = 12. (I) Fluorescent imaging of lyz: DsRed-positive cells in cyclophosphamide-treated Tg(lyz: DsRed) transgenic zebrafish at 6 dpf. (J) Quantification of lyz: DsRed-positive cells in each group. n = 6. Data are expressed as the mean ± SEM. **p < 0.01; ***p < 0.001; ****p < 0.0001 versus the model group.