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Fig. 4 - Supplemental 1 id2b-/- larvae exhibit a decreased number of valve endocardial cells while maintaining normal atrioventricular valve function. (A) Representative confocal images of valve endocardial cells in 96 hr post-fertilization (hpf) id2b+/+ and id2b-/- hearts carrying Tg(kdrl:nucGFP). (B) Quantification of the number of valve endocardial cells in id2b+/+ and id2b-/- hearts. VECs, valve endocardial cells. n=(10, 10). (C) Representative confocal images of 72 and 120 hpf id2b+/+ and id2b-/- hearts in Tg(kdrl:nucGFP); Tg(myl7:mCherry) transgenic background. (D) Statistical analysis of the number of endocardial cells in the ventricle (V), atrium (A), and combined (A+V) in id2b+/+ and id2b-/- hearts. n=(13, 13) (72 hpf); n=(12, 15) (120 hpf). (E) Quantification of blood flow patterns in 96 hpf id2b+/+ (n=16) and id2b-/- (n=15) hearts. (F) Hematoxylin and eosin (HE) staining of adult id2b-/- and id2b+/+ hearts after echocardiographic analysis in Figure 4F and G. Enlarged views of boxed areas are shown in the bottom panels. Data are presented as mean ± s.e.m. p-values were calculated by unpaired two-tailed Student’s t-tests. ****p<0.0001. ns, not significant. Scale bars, 20 μm (A), 50 μm (C), 200 μm (F).