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Fig. 2 - Supplemental 1 Expression pattern of EGFP in the sox17::egfp transgenic testis. Staining of sox17::egfp testis sections with anti-GFP antibody (magenta) and DAPI (cyan). Note that EGFP was strongly expressed in the 1- to 2-cell stage spermatogonia and weakly expressed in the 4- to 8-cell spermatogonia and started to fade at the 8-cell spermatogonia. Stem cell function of sox17::egfp spermatogonia was already confirmed by transplantation experiment (Kawasaki et al., 2016). Dotted lines indicate cysts containing the cell types indicated above in the image. Scale bar: 10 µm.
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