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Fig. 1 - Supplemental 2 Expression patterns of Meioc in wild-type germ cells. (A) Expression patterns of meioc mRNA and Meioc protein in gonads at 25 days post-fertilization (dpf) and adult testes and ovaries. Black arrowheads indicate cells that express neither mRNA nor protein, and white arrowheads indicate cells that have the mRNA signals and weak protein signals. Yellow dotted lines indicate cysts containing cells positive for both mRNA and diffuse protein signals, including small or weak granules, and green dotted lines indicate cysts containing cells with mRNA signals and bright protein signals, including large granules. Scale bar: 10 µm. (B) Double staining of ovary at 25 dpf with anti-Meioc antibody (green) and anti-Sycp3 antibody (red). Staging of oocytes was defined by the patterns of Sycp3. Scale bar: 5 µm. (C) Double staining of wild-type testes with anti-Meioc antibody (green) and anti-Sycp3 antibody (red). Staging of spermatogonia was defined by the number of cells in the cyst, and staging of spermatocytes was defined by the patterns of Sycp3. Scale bar: 5 µm.