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Fig. 1 Low translational activity of meioc mutant spermatogonia. (A) Schema of the development of spermatogonial cysts surrounded by Sertoli cells and progression of spermatogenesis in zebrafish. (B) Histology (HE) and immunostaining against Plzf and spermatocyte markers (Sycp1, 3) in the wild-type and the moto-/- testes. Arrowheads: sperm. SC: spermatocytes. Scale bar: 10 µm. (C, D) OP-Puro fluorescence analysis (C) and quantification of the signal intensities (D) in wild-type and meiocmo/mo spermatogenic cells. Dotted lines: 1- to 2-cell spermatogonia. Scale bars: 10 µm. (E–F) Effect of cycloheximide (CHX, 0.2 µM) on differentiation of spermatogonial stem cells (SSCs) in culture. Dotted lines: germ cell clumps. Sycp3: immunostaining of Sycp3. Arrowheads: examples of a cell with a large nucleolus. Scale bar: 50 µm. The graph (F) presents the percentage of clumps of SSCs and differentiated cells (Differ) shown in E. Indistinguish: not determined whether dominant cell type was stem or differentiated. Data are represented as mean ± SD. *p<0.05, **p<0.01.