ZFIN Image: Eckert et al., 2025, Figure 5

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Figure 5

Transcriptional profiling reveals adora2ab regulation in the Omarigliptin-treated nephronophthisis model. (a) Principal component analysis (PCA) of the Log2CPM count data shows the distinct clustering of DMSO- and Omarigliptin-treated nphp1/nphp4 morphant samples. (b) Volcano plot of the RNA sequencing data analysis comparing nphp1/nphp4 morphant embryos treated with Omarigliptin versus DMSO treatment reveals significant upregulation of adora2ab. (c) Gene set enrichment analysis identifies cilium-associated processes among the top 10 upregulated pathways (by adjusted p-value) in Omarigliptin-treated nphp1/nphp4 morphant embryos. (d) Violin plot showing the log₂FC of adora2ab expression from RNA sequencing data, comparing nphp1/nphp4 morphant embryos treated with Omarigliptin versus DMSO treatment. Adj. p-value is shown. (e) Whole-mount in situ hybridization showing spatial expression pattern of adora2ab at 24 hpf and 48 hpf. Bar = 250 µm. (f) RT-PCR analysis showing the temporal expression profile of adora2ab from the 16-somite stage to 3 dpf, with ef1a serving as a loading control.

Acknowledgments

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