Fig. 5

Fig. 5

DNAJC7 is required for the disassembly of stress-induced TDP-43 condensates. a Representative living-cell images showing TDP-43^ΔNLS-Clover condensates induced by arsenite treatment for 1 h, followed by the removal of stress in U2OS cells treated with control siRNA, or siRNAs targeting HSPA1A, HSPB1, BAG2, and DNAJC7. b Percentage of cells forming TDP-43 condensates after the 1-h arsenite treatment. Data represents means from four independent experiments with individual values shown. c Percentage of cells in which TDP-43 condensates disassemble after 12 h of stress removal. Data represents means from four independent experiments with individual values shown. **p < 0.01 (non-repeated measures analysis of variance and Bonferroni post hoc test). d Representative live-cell images showing TDP-43^ΔNLS-Clover condensates induced by arsenite treatment for 1 h, followed by stress removal in U2OS cells transduced with DNAJC7-mCherry lentivirus to increase DNAJC7 levels. e Percentage of cells forming TDP-43 condensates after 1-h arsenite treatment. Data are shown as mean ± SD from two independent experiments. *p < 0.05 (two-tailed unpaired Student’s t-test). f Percentage of cells recovering and disassembling TDP-43 condensates after 12 h of stress removal. Data are shown as mean ± SD from two independent experiments. *p < 0.05 (two-tailed unpaired Student’s t-test)