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Fig. 5 (a) Fluorescence inverted microscope imaging of NAHCP (10 μM) in living zebrafish. Top row: zebrafish was incubated with NAHCP (10 μM) for 30 min as the control group; middle row: zebrafish was incubated with NAHCP (10 μM) for 30 min and then with H2S 50 μM for 30 min; bottom row: zebrafish was incubated with LPS (1.0 μg mL−1) for 2 h and then with NAHCP (10 μM) for 30 min. (b) The quantitative analysis of the average fluorescence in the top row, middle row, and bottom row. λex = 420 nm, λem = (520–550) nm, scale bar: 10 μm, n = 3.
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