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Fig. 5 (A–I) Cholesterol synthesis and transport gene transcription are significantly affected by exposure to AY9944 and some psychopharmaceuticals. RT-qPCR was used to measure fold change in mRNA transcription relative to the house keeping gene Actin. (A–C) are genes associated with cholesterol synthesis prior to the Bloch and Kandutsch-Russell pathways. (D–G) are genes associated with cholesterol synthesis in the Bloch and Kandutsch-Russell pathways. (H,I) are cholesterol transport genes. N ≥ 3, 30 fish per condition for each replicate. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, determined by one-way ANOVA followed by Tukey’s post hoc HSD. VEH = 0.1% DMSO, AY9944 = positive control, CAR = Cariprazine, ARI = Aripiprazole, TRZ = Trazodone. Error Bars = 95% confidence intervals.