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Fig. 4 (A–F) Sterol deposition in zebrafish following exposure to AY9944 and psychopharmaceuticals. Sterols were imaged using filipin immunohistochemistry. (A) VEH treated zebrafish. White outline indicates the area of the eye measured for quantification of filipin intensity. (B) AY9944 treated zebrafish. (C) CAR treated zebrafish. (D) ARI treated zebrafish. (E) TRZ treated zebrafish. (F) Relative filipin fluorescent intensity (Fluorescent Unit/Area) of the eye all treatments. Images were taken at a magnification of 10.5X. Scale bar = 221 µm. N ≥ 4. * p < 0.05, ** p < 0.01, *** p < 0.001, determined by one-way ANOVA followed by Tukey’s post hoc HSD. VEH = 0.1% DMSO, AY9944 = positive control, CAR = Cariprazine, ARI = Aripiprazole, TRZ = Trazodone. Error Bars = 95% confidence intervals.