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Fig. 2 (A–F) LC-MS/MS analysis of cholesterol precursors and cholesterol following exposure to AY9944 and psychopharmaceuticals. Cholesterol precursor and cholesterol levels were measured in AY9944, CAR, ARI, and TRZ-treated zebrafish. Results are presented as relative fold change normalized to the vehicle (VEH)-treated zebrafish. (A) Lanosterol, (B) 7-DHC, (C) cholesterol, (D) desmosterol, (E) 7-DHC/cholesterol, (F) desmosterol/cholesterol. N ≥ 3 trials with at least three technical replicates, 30 fish per condition for each trial. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, determined by one-way ANOVA followed by Tukey’s post hoc HSD. VEH= 0.1% DMSO, AY9944 = positive control, CAR = Cariprazine, ARI = Aripiprazole, TRZ = Trazodone. Error Bars = 95% confidence intervals.