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Fig. 14 Effect of TZP on protein expression of GSK-3β and total AMPK level in zebrafish in old and young age group, A GSK-3β; B AMPK activity. After the treatment of TZP, the level of AMPK was considerably increased in both age groups as compared to HFD group and GSK-3β was reduced in TZP-treated groups. The statistical data analysis is presented by two-way ANOVA analysis by employing the Bonferroni post-test. Values presented as mean ± SD (p < 0.05), a vs. control, TZP (10 nM) per se, a vs. HFD, b vs. HFD + TZP (10 nM), c vs. HFD + DPZ (5 mg/kg). Values presented as mean ± SD (p < 0.05), α vs. HFD (young age group), β vs. HFD + TZP (young age group) (n = 3). A [F (4, 20) = 8.86, p = 0.0003], B [F (4, 20) = 1.63, p = 0.2065]