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Publication: Ingham et al., 2025 - Loss of SLX4IP leads to Common Fragile Sites instability and compromises DNA interstrand crosslink repair in vivo
All Figures: Figures for Ingham et al., 2025

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Fig. 1 SLX4IP localizes to stressed CFSs. A, representative images of SLX4IP foci. SLX4IP forms distinct nuclear foci induced by APH (0,2 μM). B, for cell cycle analysis, U2OS cells are sorted by mean EdU and total DAPI content. This allows for quantification of SLX4IP foci in each cell cycle stage (mean ± SD; n = 3 biological replicates; >260 cells analyzed per condition). C, representative images of SLX4IP colocalization with FANCD2 in U2OS prometaphase cells. D, quantification of foci in (C) (mean ± SD; n = 3 biological replicates; 50 cells analyzed per condition). E, ChIP-qPCR analysis of GFP-SLX4IP or GFP localization to GAPDH or FRA7H in cells treated with DMSO or APH (0,2 μM) (mean ± SD; n = 2 biological replicates). Significance was assessed by Student’s unpaired t-tests. Scale bars: 10 μM.

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