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Fig. 5 Expression of CFBE cells pro-inflammatory genes in response to mono- and co-infections. Expression of IL1B, IL8, TNFα and IL6 genes was measured using RT-qPCR and normalized with the expression of GAPDH and RPS18 genes. RNA was extracted from CFBE cells after 6 h of infection. (A) Inflammatory response upon mono-infections. (B) Inflammatory response caused by PA190A and PA190B in the context of mono- or co-infections. Results from panels A and B were collected in the same experiments and the same dataset was used for mono-infections with PA190A and PA190B in both panels. Data are presented as FC of gene expression of infected cells vs. non infected cells with FC = 2^(−ΔΔCT). Values are the means ± standard error of the mean (SEM) of four replicates. Statistical analyses were done using a Kruskal-Wallis test followed using a Dunn test. *P < 0.05.