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Fig. 4 Expression of zebrafish pro-inflammatory genes in response to mono- and co-infections. Expression of il1b, il8, tnfa and il6 genes was measured using RT-qPCR and normalized with the expression of ef1a gene. RNA was extracted from 10 larvae after mono- or co-infections at 3 hpi and 20 hpi. (A) Inflammatory response upon mono-infections. (B) Inflammatory response caused by PA190A and PA190B in the context of mono- or co-infection. Results from panels A and B were collected in the same experiments and the same dataset was used for mono-infections with PA190A and PA190B in both panels. Data are presented as fold change (FC) of gene expression in infected larvae vs. non infected with FC = 2^(−ΔΔCT). Values are the means ± standard error of the mean (SEM) of four replicates (n = 4 from pools of 10 larvae per timepoint, 40 larvae in total). Statistical analysis was done using a one-way ANOVA followed by a Tukey post-hoc test. *P < 0.05; **P < 0.01; ***P < 0.001.