Fig. 3.

Fig. 3. Foxh1 and Ets sites of the hemichordate SFZE syntax are functionally important for notochord activity.

(A) Diagram of the reporter constructs of PfCRM2 with deletions of the Foxh1 sites or mutations of the Ets sites. The additional Foxh1 site in the vector is indicated by the leftmost blue arrow with black outline. (B to G) Representative images show zebrafish embryos injected with the truncated or mutated PfCRM2 reporter constructs. Magnifications of the notochord regions highlighted by white boxes in (B), (D), and (F) are shown in (C), (E), and (G), respectively. EGFP signals in the notochord cells are indicated with magenta arrowheads. Embryos are oriented in the dorsal view with anterior to the top. Top panels [(B), (D), and (F)] and bottom panels [(C), (E), and (G)] are in the same scale as indicated in (B) and (C), respectively. w/o, without; delta symbol (△), mutation. All scale bars represent 100 μm. (H) Percentages of zebrafish embryos exhibiting EGFP signals in the notochord among EGFP-positive embryos. Each yellow data point represents the result of a single experiment. The gray columns are average results from at least three biological replicates, with error bars showing SDs. *P < 0.05; ****P < 0.0001.