ZFIN Image: Zhao et al., 2025, Fig. 2

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Figure Caption

Fig. 2

Spatiotemporal Expression and mutant Establishment of Zebrafish foxo1a

A: Single-cell RNA sequencing data of early zebrafish development, (a) high temporal resolution single-cell RNAseq time course, covering embryogenesis and early larval development. (b) UMAP projection of single-cell transcriptomes colored by selected major tissues. (c) foxo1a single-cell RNA data during early development.

B: Early developmental single-cell data of foxo1b, olig2, mbp, plpa, gfap and s100b.

C: Schematic diagram of foxo1a mutant establishment.

D: Schematic diagram of the foxo1a mutant target. The deletion of 8bp (AGCTCCGC) in the first exon results in a frameshift mutation.

E: HRMA of the foxo1a gene, sequencing chromatograms for genotyping wild-type, foxo1a heterozygous, and homozygous mutants.

Acknowledgments

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