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Fig. 1 Targeting of bmp7b and bmpr1ba with CRISPR/Cas9 results in cyclopia. (A) Scheme of experimental procedure. Embryos were injected at 1-cell stage and analyzed at approximately 24–48 hpf. (B) Wild-type, dorsal view. (C) bmp7b Crispant with cyclopia, ventral view. (D) bmpr1ba Crispant with cyclopia, ventral view. Scalebars indicate 200 µm. (E) Pie charts show the frequency of phenotypes in surviving embryos in bmp7b and bmpr1ba Crispants.
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