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Fig. 2

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Publication: Herdt et al., 2025 - In vivo measurement of an Apelin gradient with a genetically encoded APLNR conformation biosensor
All Figures: Figures for Herdt et al., 2025

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Fig. 2 Pharmacological characterization of the APLNR-cpGFP biosensors.

Stimulation with Apelin and Apela (arrow indicates timepoint of ligand addition) led to a significant increase of APLNR(F233)-cpGFP (a, c) and APLNR(K235)-cpGFP (b, d) biosensor fluorescent intensity. The APLNR antagonist ML-221 significantly diminished the Apelin-induced fluorescent response of APLNR(F233)-cpGFP (e) and APLNR(K235)-cpGFP (f). Data are presented as mean values ± SEM from three independent experiments conducted in stable APLNR-cpGFP expressing HEK293T cells. cpGFP circularly permuted GFP, HEK293T human embryonic kidney 293T. Source data are provided as a Source Data file.

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