Fig. 8 Histology and ultrastructure of the injury area in ankrd1a mutant and wild-type (wt) skeletal muscle at 5 days postinjury (dpi). Representative light microscopy (LM) images of ankrd1a+/+ (A) and ankrd1a−/− (B) skeletal muscle (n = 4). Muscle fibers are blue, collagen is pink. C–F: representative transmission electron microscopy (TEM) images of ankrd1a+/+ (C and D) and ankrd1a−/− (E and F) skeletal muscle. G–I: autophagic vesicles observed in ankrd1a+/+ (G) and ankrd1a−/− (H and I) skeletal muscle. J: macrophage detected in ankrd1a−/− skeletal muscle. Av and *, autophagy vesicle; ECM, extracellular matrix; M, myofiber; m, newly growing myofiber; Mb, myoblast; Mφ, macrophage. Scale bars, 20 µm for A and B and 1 µm for C and J.
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