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Fig. 1 Upregulation of endogenous ankrd1a expression in injured adult zebrafish skeletal muscle correlates with the expression of genes activated during several phases of skeletal muscle repair. mRNA levels of ankrd1a and ankrd1b (A) and representative marker genes involved in the activation of satellite cells (pax7a), myoblast proliferation (myod1 and pcna), and myocyte differentiation [myogenin (myog) and myosin heavy chain isoforms 1.1 (myhz1.1) and 2 (myhz2)] (B) were assessed at 3, 5, 7, and 10 days postinjury (dpi) by RT-qPCR. At least 3 zebrafish were used per experimental group. RNA isolated from the skeletal muscle of noninjured zebrafish served as a control (NI). The data for A are presented as fold change ± SD, normalized to the ankrd1a expression level in noninjured control. The data on B are presented as mean log2 fold change ± SD. The exact P values are given in Supplemental Table S8. Average Ct values ± SD are listed in Supplemental Table S3. MuSC, muscle satellite cells. *P ≤ 0.05.